Scientific research

Action anticancérigène de l'Huile Essentielle d'Origanum Compactum : application sur la lignée cancéreuse humaine du nasopharynx "Hep"

L'Origan -Origanum compactum- est une plante médicinale couramment utilisée pour certains traitements thérapeutiques notamment des affections gastriques et cutanées; elle montre aussi un intérêt majeur en tant que produit aromathérapeutique. Dans ce sens; nous nous sommes intéressés à l'évaluation de ses actions antigénotoxique et anticarcinogène contre certains agents mutagènes de référence comme l'éthyle carbamate (Uréthane). Nos résultats montrent un taux d'inhibition des clones allant de 59% jusqu'à 63% pour certaines fractions de l'Origanum compactum chémotype marocain étudiées par test de mutations et de recombinaisons somatiques (SMART) au niveau des ailes de Drosophila melanogaster. Télécharger le résumé Read more...

Effect of essential oils on the in vitro growth of penicillium digitatum and penicillium italicum infecting citrus and colletotrichum musea infecting banana

Citrus and bananas represent the two economically important fruit crops worldwide. The most important causes of post-harvest losses of these fruits are blue mold caused by Penicillium italicum and green mold caused by Penicillium digitatum for citrus, and anthracnose disease caused by Colletotrichum musea for bananas. To cope with these diseases, treatment with chemical fungicides during the conditioning process has long been used. Nowadays, such a treatment is challenged because of the following reasons: High level of chemical fungicide residues in food products Chemical fungicides toxicity and risk for human health and for the environment, Development of pathogens populations resistant to chemical fungicides, Restriction or ban on use of a number of chemical fungicides by the legislation. This has motivated the search for alternative approaches. Essential Oils (EOs) extracted from aromatic plants are known for their antifungal effects and could thus be used against plant pests. The present work was therefore undertaken to study the effect of ten Eos on the in vitro growth of P. italicum, P.digitatum and C. musea. Télécharger le poster Read more...

The impact of oregano (origanum heracleoticum) essential oil and carvacrol on virulence gene transcription by escherichia coli O157:H7

Enterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7was first recognized as a food pathogen in 1982 (Paton and Paton 1998). Since then, this organism has been implicated in sporadic cases and outbreaks of enterohaemorrhagic diarrhea worldwide (EFSA and ECDC 2013; Crim et al., 2014). EHEC O157:H7 is a member of the attaching and effacing (A/E) E. coli group. It possesses virulence factors essential for adhesion of the pathogen to intestinal epithelial cells (attachment) and is responsible for the destruction of the brush border ofmicrovilli (effacement) (Paton and Paton 1998). Télécharger l'étude complète Read more...

Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria

Pathogenic and food spoilage bacteria have been considered as the primary causes of food-borne diseases and food quality deterioration in both developed and developing countries. In order to assure the food safety and to extend the shelf life of food products, additions of chemical preservative agents into food products or decontamination treatments via physical, chemical or biological process or their combinations have been widely applied in food industries (Brul and Coote 1999; Gould 2000). However, critical concerns have been raised due to limitations of treatment processes and since survival of environment-adapted bacteria after treatment processes may lead to high resistance of bacteria such as pathogenic Escherichia coli O157:H7, Listeria monocytogenes, and some Salmonella serovars (Whitney et al. 2007; Hugas and Tsigarida 2008; Rajkovic et al. 2009). Télécharger l'étude complète Read more...

Modifications fonctionnelles et ultrastructurales des cellules de Pseudomonas aeruginosa et Staphylococcus aureus induites par l'huile essentielle de Cinnamomum verum

Functional and ultrastructural changes in pseudomonas aeruginosa and staphylococcus aureus cells induced by cinnamomum verum essential oil

Microbial spoilage is the most common cause of food deterioration. Thus, one of the major concerns of the food industry is the control of spoilage and pathogenic organisms. Therefore, a broad range of chemicals that inhibit microbial growth are added to food during manufacture to extend shelf-life and ensure food safety and quality. However, although the effectiveness of traditional preservatives is recognized, their safety has been questioned (Türkoglu 2007). Download the full study Read more...

Genotoxicity and antigenotoxicity of some essential oils evaluated by wing spot test of drosophila melanogaster

Essential oils extracted from the three medicinal plants; Helichrysum italicum, Ledum groenlandicum and Ravensara aromatica, together with their mixture were tested for their genotoxic and antigenotoxic activities against urethane, a well-known promutagen.We have adopted the somatic mutations and recombination test (SMART) in the wings of Drosophila melanogaster. Three days old larvae, trans-heterozygous for two genetic markers mwh and flr, were treated by essential oil and/or urethane. A negative control corresponding to solvent was also used. Télécharger l'étude complète Read more...

Effets antigénotoxiques de trois huiles essentielles chez la levure diploïde (Saccharomyces cerevisiae) après traitement par rayonnement UVC

Antigenotoxic effects of three essential oils in diploid yeast (saccaromyces cerevisias) after treatments with UVC radiation

Essential oils (EOs) extracted from medicinal plants such as Origanum compactum, Artemisia herba alba and Cinnamomum camphora are known for their beneficial effects in humans. The present study was undertaken to investigate their possible antigenotoxic effects in an eukaryotic cell system, the yeast Saccharomyces cerevisiae. The EOs alone showed some cytotoxicity and cytoplasmic petite mutations, i.e. mitochondrial damage, but they were unable to induce nuclear genetic events. In combination with exposures to nuclear mutagens such as 254-nm UVC radiation, 8-methoxypsoralen (8-MOP) plus UVA radiation and methylmethane sulfonate (MMS), treatments with these EOs produced a striking increase in the amount of cytoplasmic petite mutations but caused a significant reduction in revertants and mitotic gene convertants induced among survivors of the diploid tester strain D7. Download the full study Read more...

Antibacterial and antioxydant activities of origanum compactum essential oil

In the present study, essential oil of Origanum compactum was analysed and its chemical composition was identified by gas chromatography coupled to mass spectrometry (GC-MS). Among thirty two assayed constituents, carvacrol (30.53%), thymol (27.50%) and its precursor g-terpinene (18.20%) were found to be the major components. The oil was investigated for its in vitro antibacterial activity against a panel of standard reference strains using well diffusion and broth dilution methods. In solid medium, the oil was found to be remarkably active against all tested strains except Pseudomonas which showed resistance. Télécharger l'étude complète Read more...

Aromatherapy alternatives for gynaecological pathologies : recurrent vaginal candida and infection caused by the human papilloma virus

Scientific aromatherapy and integrated medicine are taking an increasingly greater role in the treatment of a number of infectious diseases for which conventional medicine has few effective solutions. Powerless and faced with treatment failure, some therapists are actively seeking alternative effective active substances to resolve this inadequacy. When prescribed by competent scientists, essential oils that are chemically and chemotypically defined provide real opportunities with encouraging measurable results that can be observed in clinical practice. Télécharger l'étude complète Read more...

Screening of antifungal and antibacterial activity of 90 commercial essential oils against 10 pathogens of agronomical importance

Nowadays, the demand for a reduction of chemical pesticides use is growing. In parallel, the development of alternative methods to protect crops from pathogens and pests is also increasing. Essential oil (EO) properties against plant pathogens are well known, and they are recognized as having an interesting potential as alternative plant protection products. In this study, 90 commercially available essential oils have been screened in vitro for antifungal and antibacterial activity against 10 plant pathogens of agronomical importance. Télécharger l'étude complète Read more...

Activités antibactériennes et cytotoxiques de dix huiles essentielles disponibles dans le commerce

Antibacterial and cytotoxic activities of ten commercially available essential oils

There is a huge concern in the medical field concerning the emergence of bacterial resistance to antibiotics. Essential oils are a source of antibacterial compounds that can overcome this problem. Ten essential oils that are commercially available were investigated in the present study: ajowan, basil, German chamomile, Chinese cinnamon, coriander, clove, lemongrass, Spanish lavender, oregano and palmarosa. Their direct, synergistic and indirect antibacterial activities were evaluated against different human pathogenic Gram-positive and Gram-negative strains. To evaluate their possible use in clinics, the cytotoxicity of these essential oils was also tested on keratinocyte and epithelial cell lines. Except for the Chinese cinnamon, coriander and lemongrass, all other essential oils presented no cytotoxicity at 32 and 16 µg/mL. Download the full study Read more...

Évaluation des propriétés bactériostatiques des huiles essentielles et de leurs applications potentielles en microbiologie alimentaire

Evaluation of the bacteriostatic properties of essential oils and their potential applications for food microbiology

The recent multiplication of food scandals has made food safety a major concern for food producers. More than ever, consumers are asking for products that are microbiologically safe and, in the same time, containing less artificial preservatives. The use of small concentrations of essential oils could bring a solution to this dilemma, as some of those “natural additives” already have proven bacteriostatic properties. Essential oils also beneficiate from a very good image, thanks to their numerous properties (antioxidant, antiviral, anti-inflammatory) and the fact they have been used by humans for thousands of years. Download the full study Read more...

Genotoxicity of some essential oils frequently used in aromatherapy

Genotoxic properties of the essential oils extracted from Artemisia dracunculus (tarragon), Ocimum basilicum (basil), Cinnamomum loureirii (cinnamon), Laurus nobilis (laurel), Satureja montana (savory) and Rosmarinus officinallis (rosemary) are studied by Drosophila melanogaster Somatic Mutation and Recombination Test (SMART). The high bioactivation crossed with a high cytochrome P450-dependent bioactivation capacity is used. This assay is principally based on the loss of heterozygosity of the suitable recessive markers’ multiple wing hairs (mwh) and flare-3 (flr3) which can lead to the formation of mutant clones of larval cells, and which are then going to be expressed as spots on the wings of adult flies. Third-instar larvae are treated for 48 hr with different concentrations of the essential oils dissolved in Tween-80 at 0.2% or 2%. The wings of the emerging adults are analyzed for the occurrence of different types of mutant spots. No statistically significant differences in spot frequencies between negative controls and treated series are observed. These results suggest that the six essential oils at concentrations tested are not genotoxic towards somatic cells of D. melanogaster. Télécharger l'étude complète Read more...

Retrouver l’odorat avec les huiles essentielles ?

Recover your sense of smell with Essential Oils?

Did you know that among the most common symptoms of COVID-19 (fever, cough, body aches, etc.), a European study revealed that 85% of patients suffering from a mild form of the infection complained of difficulty smell and 89% of taste alteration. Want to know more about loss of smell (= anosmia) and essential oils? Read more...

Arrêter de fumer à l'aide des huiles essentielles

Stop smoking using Essential Oils

Period illness or illness? Addiction is a problem that gets people talking! Addictions in our society are numerous and essential oils can be real allies to help us quit smoking naturally. In this tobacco-free month, focus on the most widespread addiction in the world. So, are you ready to put out your last cigarette? Read more...

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